福州市三县洲大桥斜拉桥边跨现浇施工

该斜拉桥主跨箱梁系分块预制拼装法施工 ,边跨箱梁系分段现浇施工 ,本文着重介绍边跨现浇施工方法及相关的工艺措施     

ZR10(12)型养蚕煤炉的研制

针对普通煤炉上火慢、热效率低等缺点,用增加造汽箱、喷汽盘等方法,改进设计了一种新型的养蚕专用煤炉。养蚕煤炉的工作原理是:输入适量水至造汽箱腔里,在炉胆余热作用下,能很快转化为蒸汽流,由喷汽盘12小孔注入煤孔内,与煤燃烧产生的一氧化碳和二氧化碳发生热解反应,生成甲烷类汽体,成为一种高能燃料,其着火能量低,燃烧速度快,发热值高,极易形成火焰,还可生成可燃性汽体H2、O2、CO,使燃烧更加完全,让蚕在适温适宜环境中生长、成熟。与普通煤炉相比较,养蚕煤炉可节煤30%以上;一氧化碳平均值220×10-6L/L以下;造汽箱容量1 L,进水、喷汽孔径0.7 mm,火焰射高300 mm;省时30%以上。     

制订生物制品冻干曲线的探讨

探讨了生物制品冻干过程中,不同阶段油温、板温、苗温、箱温的变化规律,并将其同冻干时间有机结合起来,用于制订冻干曲线。     

大鳞副泥鳅Sox基因保守区的克隆与特征分析 

为探讨大鳞副泥鳅(Paramisgurnus dabryanus)的性别决定与分化机制,采用Sox基因HMG盒保守区的2对简并引物,SoxN与Sox9,用以扩增大鳞副泥鳅的基因组DNA,并对其性腺组织进行RT-PCR分析。SoxN引物扩增基因组DNA共获得pdSox1、pdSox2、pdSox3、pdSox4、pdSox11、pdSox14、pdSox19、pd-Sox21等13个Sox基因,分属SoxB与SoxC类群;Sox9引物扩增基因组DNA共获得pdSox8、pdSox9等5个Sox基因,属于SoxE类群,这5个Sox基因均含有一个内含子,且内含子的起始位置一致。基于Sox基因HMG盒蛋白质的遗传距离构建的NJ系统进化树表明,大鳞副泥鳅18个Sox基因在小鼠或斑马鱼中均能找到相应的Sox直系同源基因,其中,pdSox4、pdSox8、pdSox9、pdSox11、pdSox14、pdSox19等6个基因存在基因加倍现象,具有2个或2个以上的拷贝。用Sox9引物对大鳞副泥鳅的性腺进行RT-PCR扩增和测序分析,结果表明,在精巢中表达的是pdSox9a,在卵巢中表达的是pdSox8a。以上结果表明,pdSox8与pdSox9是大鳞副泥鳅的性别相关基因,它们可能在大鳞副泥鳅的性别分化中发挥作用。     

不同包装材料对绿芦笋保鲜效果的影响

研究了低温条件下贮藏12 d不同包装材料对绿芦笋的保鲜效果。结果表明,微孔膜包装可明显抑制绿芦笋的呼吸强度,降低水分损失和叶绿素的分解速率,较好地保持了绿芦笋的原有品质;PE膜包装能够有效降低VC的降解速率,但保鲜效果较微孔膜次之;保鲜纸包装能够较好地抑制叶绿素的分解,但贮藏期较短;泡沫箱包装对延缓绿芦笋衰老效果不明显,不适于用作绿芦笋贮藏保鲜的包装材料,可结合其他措施作为周转箱使用。     

The effect of Astragaloside IV on immune function of regulatory T cell mediated by high mobility group box 1 protein in vitro

High mobility group box 1 protein (HMGB1), a potent pro-inflammatory cytokine, contributes to the pathogenesis of diverse inflammatory and infectious disorders. Some studies have illustrated the potential effect of HMGB1 on regulatory T cells (Tregs). Astragaloside IV (AST IV) isolated from a Chinese herb, Astragalus mongholicus, is known to have a variety of immunomodulatory activities. However, it is not yet clear whether AST IV possesses potential regulatory effect on the pro-inflammatory ability of HMGB1 with subsequent activation of Tregs. This study was carried out to investigate the antagonistic effects of different doses of AST IV on the immune function of Tregs mediated by HMGB1 in vitro. Tregs isolated from the spleens of mice were co-cultured with HMGB1 and/or AST IV. Cell phenotypes of Tregs were analyzed, and the contents of various cytokines in the cell supernatants as a result of co-culture and the proliferation of CD4⁺CD25⁻ T cells were determined. Results showed that HMGB1 stimulation resulted in significantly down-regulation of expressions of Tregs cell phenotypes. However, AST IV can rival the suppressing effect of HMGB1 on immune function of Tregs with a dose-dependent in vitro. These results indicate that AST IV has the potential therapeutic action on inflammation augmented by HMGB1.     

Sensitivity Analysis of Cantilever Box Beam Temperature Effects Based on the Prestressed Concrete Parameter Changes

On the construction of large-span prestressed box beam Cantilever, the parameter frequent changes in concrete modulus E, coefficient of thermal expansion α.In a certain temperature case, the change of concrete parameters will cause the changes on internal stress and strain of the bridge and the difference of the changes will affect the bridge safety. Accordingly,based on a bridge cross-section of a temperature field and combined with the actual bridge construction, give concrete Cantilever bridge parameters change, calculated and analyzed the sphere of influence of parameters on the stress and strain fields of bridge, asked the law of sensitivity on stress changes. Summarize the sensitivity factors of temperature stress which is used to improve bridge safety.     

小麦WPBF与高分子量谷蛋白基因上游Prolamin-Like box的特异结合

【目的】克隆普通小麦胚乳特异表达转录因子基因WPBF,对其原核表达条件进行优化,并验证其编码蛋白和小麦高分子量谷蛋白亚基基因（HMW-GS）上游Prolamin-Like box的结合特性,为小麦HMW-GS表达调控机制的阐明奠定基础。【方法】利用RT-PCR扩增得到WPBF编码区,将其连接到pET-21a-MBP载体中,并分别在E.coli T7 Express和Origami B（DE3）菌株中诱导表达。依次利用Amylose和Ni-NTA亲和层析柱纯化重组蛋白,最后通过凝胶迁移阻滞试验（EMSA）对重组蛋白的DNA结合特性进行分析。【结果】在33℃的Origami B（DE3）菌株中表达含MBP和His双标签的重组WPBF大部分以可溶性形式存在,其表达量占细菌总蛋白的48.0%。重组WPBF和含HMW-GS上游Prolamin-Like box的两类DNA探针能发生特异结合,但重组WPBF和含“TGCAAAG”基序DNA探针的结合强度高于其和含“TGCAAG”基序DNA探针的结合。【结论】建立了优化的WPBF原核表达系统,并获得具有生物活性的重组蛋白;重组WPBF能与HMW-GS启动子来源的两种Prolamin-Like box特异性结合, 但结合能力存在差异。     

核因子Y在植物中的分类及其功能概述

核因子Y(NF-Y)是近十年来新发现的具有多种生物功能的植物特异转录因子,由三个不同的亚基组成,分别是NF-YA(CBF-B/)HAP、NF-YB(CBF-A/HAP3)和NF-YC(CBF-C/HAP5)。NF-Y是一种典型的通过与调控因子相互作用来调控下游基因表达的转录因子,通过保守专一性的序列与真核生物启动子区域的CCAAT框相结合。NF-Y在植物中普遍存在,并参与调节植物体的诸多重要生理过程,包括逆境反应,胚胎和叶绿体发育,光合作用等重要生理过程。文章主要就植物NF-Y转录因子的基本结构特征、生物学功能、研究方法及进展等进行了综述。     

箱涵进海路结构的设计研究

箱涵进海路结构是一种适用于软土海底地基上的轻型海上路基结构,具有承载能力较好,施工方便快捷的特点。本文结合埕海油田张东开发海工工程,对箱涵进海路结构的抗滑、抗波浪浮托、抗倾稳定稳定进行了计算,计算结果表明箱涵进海路结构的稳定满足要求,为箱涵进海路结构的应用提供了理论上的保证。